![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K2014008:Design
Designed by: Daria Niewiadomska Group: iGEM16_UAM_Poznan (2016-10-12)
AraC-pBAD->mRFP_W
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
We were looking for the best method to optimize gene expression in E.coli.
Source
The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the mRFP permutation, with optimized codon sequence.