Part:BBa_K2014008:Design

AraC-pBAD->mRFP_W

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  

Design Notes

We were looking for the best method to optimize gene expression in E.coli.

Source

The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the mRFP permutation, with optimized codon sequence.